Abstract

CAMPOS MURILLO, Nathalie; ORELLANA BRAVO, Paola; NOGUERA CARDENAS, Patricia  and  ANDRADE TACURI, Carlos. Detection of Helicobacter pylori 23S gene involved in clarithromycin resistance . Vive Rev. Salud [online]. 2020, vol.3, n.9, pp.139-149. ISSN 2664-3243.

Abstract Introduction: One of the main factors that influence the treatment for the eradication of Helicobacter pylori is resistance to antibiotics, which differs between countries and even regions of a country. Clarithromycin is among the most widely used antibiotics for the treatment of infection. The 23S rRNA gene has been shown to be involved in resistance to this antibiotic, as a result of point mutations. Objective: To detect the mutations present in the 23S rRNA gene that encode resistance to clarithromycin in Helicobacter pylori through a non-invasive and rapid method. Materials and methods: From stool samples of 76 patients with gastrointestinal symptoms associated with the bacteria, bacterial DNA was isolated and purified, the 23S rRNA gene was identified by seminested PCR. For the detection of point mutations in the gene, RFLP was performed, using the enzymes HhaI that detects the T2717C mutation and MboII that identifies the A2142C / G mutation. Results: A total of 45 patients were positive for Helicobacter pylori, which corresponds to 59.2%. The T2717C mutation analyzed with the HhaI enzyme was present in 2.2% of the study sample, no positive results were obtained for the MboII enzyme. Conclusions: The 23S rRNA gene of Helicobacter pylori was identified through Seminested PCR, PCR-RFLP is a reliable method to detect the presence of mutations causing resistance to antibiotics, useful before choosing the eradication treatment against Helicobacter pylori infections.

Keywords : Helicobacter pylori; clarithromycin;; PCR-RFLP; mutations; Gen 23S RNA.

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