Abstract

PACHECO COELLO, Franklin. Synthesis and size estimation of silver nanoparticles, by reduction with aqueous extracts of calyces leaves and seeds of hibiscus sabdariffa linn: Promotion of green synthesis. Rev. Bol. Quim [online]. 2021, vol.38, n.3, pp.113-118.  Epub Aug 31, 2021. ISSN 0250-5460.  https://doi.org/10.34098/2078-3949.38.3.2.

The synthesis of silver nanoparticles (AgNPs) has had a positive impact on biomedical sciences, thanks to its diverse biological potentialities, among which its antimicrobial activity and in vitro interaction with various chemotherapeutic drugs stand out. The study aimed at the synthesis of AgNPs, using aqueous extracts of calyces, leaves and seeds of an organic cultivation of Hibiscus sabdariffa and a solution of silver nitrate (AgNO3). The concentration of total phenolic compounds and flavonids was determined for each extract, by the methods of Folin-Ciocalteu and Marinova respectively, this with the purpose of guaranteeing the quality and content of compounds with reducing capacity. AgNPs synthesis conditions were optimized, referring to AgNO3 volume and concentration, extract volume (calyxes, leaves and seeds), pH, heating time and temperature, using the statistical program StatGraphics. The analyzes yielded a concentration of 17.42 ± 0.12 mg GAE / g (calyces), 9.03 ± 0.91 mg GAE / g (leaves) and 11.32 ± 0.36 mg GAE / g (seeds). The maximum absorption peaks were obtained at 424 nm for all the three extracts, with absorbances of 1.5240±0.32=30-32 nm (AgNPs-calyces), 0.5674±0.24=12-14 nm (AgNPs-leaves) and 0.764 ±0.18=18-20 nm (AgNPs-seeds). The findings of the study are related to the variation of phenolic compounds in the different parts of H. sabdariffa, which conditions the synthesis performance of AgNPs. The present study represents a valuable contribution in the "Green Synthesis", representing the first study in Venezuela in which AgNPs are synthesized from H. sabdariffa.

Keywords : Nanoparticles; Reduction; Hibiscus sabdariffa; Green Synthesis; Polyphenols..

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