<?xml version="1.0" encoding="ISO-8859-1"?><article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">
<front>
<journal-meta>
<journal-id>1012-2966</journal-id>
<journal-title><![CDATA[Gaceta Médica Boliviana]]></journal-title>
<abbrev-journal-title><![CDATA[Gac Med Bol]]></abbrev-journal-title>
<issn>1012-2966</issn>
<publisher>
<publisher-name><![CDATA[Facultad de Medicina de la Universidad Mayor de San Simón]]></publisher-name>
</publisher>
</journal-meta>
<article-meta>
<article-id>S1012-29662023000200032</article-id>
<article-id pub-id-type="doi">10.47993/gmb.v46i2.608</article-id>
<title-group>
<article-title xml:lang="es"><![CDATA[Evaluación de una prueba de reacción en cadena de la polimerasa múltiplex anidada para la detección de HTLV-1]]></article-title>
<article-title xml:lang="en"><![CDATA[Evaluation of a nested multiplex polymerase chain reaction test for the detection of HTLV-1]]></article-title>
</title-group>
<contrib-group>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Serrano-Segura]]></surname>
<given-names><![CDATA[Kevin]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Miranda-Ulloa]]></surname>
<given-names><![CDATA[Eduardo]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Romero-Ruiz]]></surname>
<given-names><![CDATA[Soledad]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Cárdenas-Bustamante]]></surname>
<given-names><![CDATA[Fany]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Quiroz-Ruiz]]></surname>
<given-names><![CDATA[Hans Ramón]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
</contrib-group>
<aff id="Af1">
<institution><![CDATA[,Instituto Nacional de Salud Centro Nacional de Salud Pública Laboratorio de Referencia Nacional Virus de Transmisión Sexual]]></institution>
<addr-line><![CDATA[Lima ]]></addr-line>
<country>Perú</country>
</aff>
<aff id="Af2">
<institution><![CDATA[,Universidad Privada del Norte  ]]></institution>
<addr-line><![CDATA[Cajamarca ]]></addr-line>
<country>Peru</country>
</aff>
<pub-date pub-type="pub">
<day>00</day>
<month>00</month>
<year>2023</year>
</pub-date>
<pub-date pub-type="epub">
<day>00</day>
<month>00</month>
<year>2023</year>
</pub-date>
<volume>46</volume>
<numero>2</numero>
<fpage>32</fpage>
<lpage>36</lpage>
<copyright-statement/>
<copyright-year/>
<self-uri xlink:href="http://www.scielo.org.bo/scielo.php?script=sci_arttext&amp;pid=S1012-29662023000200032&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://www.scielo.org.bo/scielo.php?script=sci_abstract&amp;pid=S1012-29662023000200032&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://www.scielo.org.bo/scielo.php?script=sci_pdf&amp;pid=S1012-29662023000200032&amp;lng=en&amp;nrm=iso"></self-uri><abstract abstract-type="short" xml:lang="es"><p><![CDATA[Resumen El Perú es un área endémica al virus linfotrópico T humano tipo 1 (HTLV-1) y para su confirmación diagnóstica se usa pruebas serológicas que pueden dar resultados no concluyentes.  Objetivos:  evaluar una prueba de PCR múltiplex anidada para diagnosticar el HTLV-1.  Métodos:  la validación de la PCR se realizó con primers dirigidos a las regiones Pol y LTR del HTLV-1. Se empleó el gen &#946;-globina como control endógeno interno y el límite de detección se evaluó con células MT2. Los parámetros de precisión diagnóstica se evaluaron frente a 95 muestras sanguíneas de Referencia.  Resultados:  la prueba evaluada obtuvo un límite de detección de 0,5 ng/&#956;L de ADN sensibilidad diagnóstica=97,1%, especificidad diagnóstica y analítica=100%, vpn=97,2%, vpp, repetibilidad y reproducibilidad=100%; Kappa, Índice Youden=0,97.  Conclusiones:  la prueba evaluada presenta un alto rendimiento diagnóstico y debido a su bajo costo se recomienda su implementación en el algoritmo del diagnóstico de HTLV-1 en Perú.]]></p></abstract>
<abstract abstract-type="short" xml:lang="en"><p><![CDATA[Abstract Peru is an endemic area for human T-lymphotropic virus type 1 (HTLV-1) and for its diagnostic confirmation serological tests are used, which can give inconclusive results.  Objectives:  to evaluate a nested multiplex PCR test to diagnose HTLV-1.  Methods:  PCR validation was performed with primers targeting the Pol and LTR regions of HTLV-1. The &#946;-globin gene was used as an internal endogenous control and the detection limit was evaluated with MT2 cells. Diagnostic accuracy parameters were evaluated against 95 Reference blood samples.  Results:  the evaluated test obtained a detection limit of 0.5 ng/&#956;L of DNA; diagnostic sensitivity=97.1%, diagnostic and analytical specificity=100%, vpn=97.2%, vpp, repeatability and reproducibility=100%; Kappa, Youden Index=0.97.  Conclusions:  the evaluated test has a high diagnostic performance and due to its low cost, its implementation in the HTLV-1 diagnosis algorithm in Peru is recommended.]]></p></abstract>
<kwd-group>
<kwd lng="es"><![CDATA[diagnóstico]]></kwd>
<kwd lng="es"><![CDATA[retrovirus]]></kwd>
<kwd lng="es"><![CDATA[PCR anidado]]></kwd>
<kwd lng="es"><![CDATA[virus linfotrópico T tipo 1 humano]]></kwd>
<kwd lng="en"><![CDATA[diagnosis]]></kwd>
<kwd lng="en"><![CDATA[retrovirus]]></kwd>
<kwd lng="en"><![CDATA[nested PCR]]></kwd>
<kwd lng="en"><![CDATA[nested PCR nested PCR nested PCR]]></kwd>
</kwd-group>
</article-meta>
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