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Vive Revista de Salud
Print version ISSN 2664-3243
Abstract
VALLEJO PAZMINO, Gysella Imelda; ANDRANDE TACURI, Carlos Fernando; ORELLANA BRAVO, Paola Patricia and ORTIZ, Jonnathan Gerardo. Resistance of Staphylococcus aureus strains isolated in nosocomial environments. Vive Rev. Salud [online]. 2022, vol.5, n.13, pp.22-34. Epub Feb 19, 2022. ISSN 2664-3243. https://doi.org/10.33996/revistavive.v5i13.127.
Staphylococcus aureus is a microorganism with particular characteristics of virulence and resistance to antibiotics. The infections it produces occur mostly in immunocompromised individuals who may present severe consequences, despite antimicrobial therapy. Objective. To determine the resistance of Staphylococcus aureus isolated in nosocomial environments by conventional and molecular methods. Materials and methods. The study presents a quantitative, field research, observational, cross-sectional approach. Two hundred samples of isolates from nosocomial environments were analyzed by phenotypic (antibiogram by Kirby Bauer technique) and genotypic (resistance genes blaZ, mecA, and vanA by endpoint PCR) methods. Results. The results of the antibiotic susceptibility tests showed that 100% of the S. aureus strains isolated were resistant to oxacillin and penicillin g; and sensitive to vancomycin. Conclusions. the results obtained in this study showed the presence of MRSA in the different hospital areas, constituting a risk factor for horizontal transmission between health personnel and patients, which is why it is of great importance to evaluate its prevalence and establish rigorous measures for the prevention and control of its dissemination, in order to reduce the risk of new infections.
Keywords : Antibiotic Resistance; Staphylococcus aureus; blaZ; mecA; vanA.