Resumo

MONTALVO OTIVO, Jorge Manuel et al. Effect of mannitol and sorbitol on the in vitro conservation of Solanum tuberosum L. Rev. Inv. Cs. Agro. y Vet. [online]. 2025, vol.9, n.25, pp.90-104.  Epub 27-Jan-2025. ISSN 2664-0902.  https://doi.org/10.33996/revistaalfa.v9i25.334.

In vitro conservation overcomes limitations of conventional methods, offering pathogen-free plants with greater genetic uniformity. The purpose of the present research was to evaluate the effect of different concentrations of mannitol and sorbitol on the development of microtubers produced from in vitro explants of Solanum tuberosum L., in order to optimize the conditions for their conservation. A completely randomized experiment with a factorial arrangement was designed, which included three concentration levels, 0%, 2% and 4%, of both osmotic agents. The findings indicate that low concentrations, 2%, of mannitol and sorbitol favored moderate growth of the explants as osmoprotectants. However, at 4% a significant decrease in development was observed due to excessive osmotic stress. The interaction between both agents at high concentrations had a negative synergistic effect on growth. This study highlights the importance of optimizing mannitol and sorbitol in in vitro cultures to slow growth without toxicity and conserve germplasm.

Palavras-chave : In vitro preservation; Mannitol; Sorbitol; Osmoprotection; Solanum tuberosum L..

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