Abstract

VILLCA CHUQUICHAMBI, Moria; ORELLANA GUEVARA, Raquel; CONDORI MAMANI, Abigail  and  CHOQUE-MEDRANO, Juan Wilfredo. Evaluation of immunochromatographic test for the detection of IgM and IgG antibodies against SARS-CoV-2. Revista UNITEPC [online]. 2023, vol.10, n.1, pp.20-26.  Epub June 30, 2023. ISSN 2520-9825.  https://doi.org/10.36716/unitepc.v10i1.392.

Introduction: Immunological tests are used as a complementary diagnosis in epidemiological surveillance. Objectives: To evaluate the immunochromatographic test for the detection of IgM and IgG antibodies against SARS-CoV-2, using ELISA IgM/IgG as a reference test for COVID-19. Methodology: The study carried out compared IgM/IgG immunochromatographic tests with the ELISA IgM/IgG test. The estimation of sensitivity and specificity of said test was also carried out. We worked with 50 serum samples for IgM and 50 samples for IgG. Results: The sensitivity of the immunochromatographic test evaluated for the detection of IgM antibodies was 78.95% and a specificity of 48.39%. Regarding the immunochromatographic test for the detection of antibodies, IgG was 72.00% sensitive and 76.00% specific. Discussion: The agreement between the immunochromatographic test for the detection of IgM/IgG antibodies and the ELISA test for the detection of IgM/IgG antibodies shows a regular and moderate agreement according to the kappa index. The evaluation of immunochromatographic tests presents a lower sensitivity and specificity compared to other tests.

Keywords : Antibodies; COVID-19; Immunologic Tests.

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