Abstract

MAMANI-SANCHEZ, Beatriz; NOVA-PINEDO, Maximo Lucio  and  BOSQUE-SANCHEZ, Hugo. Effect of disinfection with sodium hypochlorite and multiplication with benzylaminopurine on Musa spp. RIIARn [online]. 2021, vol.8, n.1, pp.37-44.  Epub June 30, 2021. ISSN 2409-1618.  https://doi.org/10.53287/piku9019rc77e.

Within the Musaceae family, the Musa genus has acquired importance in food worldwide, either by direct consumption (fruit) or indirect (after cooking) as in the case of Bolivia, however by changing agro-economic activities this item is being affected, and also lack a mechanism for regeneration of seedlings free of diseases. The objective of the present study was to standardize the disinfection of Gran enano (banana) and Harton plantain) seedlings with different concentrations of sodium hypochlorite (NaClO) and o induce multiplication with the application of benzylaminopurine. The hyphal shoots of both varieties were subjected to two disinfection times, the first consisted of selecting the 10 to 12 cm long hyphal shoots and immersing them in 8 % sodium hypochlorite for 2 min and subsequently, were divided into two groups for the second disinfection of 2 nd 4 % with sodium hypochlorite for 7 min, then rinsed and seeded in Murashige and Skoog (MS) medium with 2 mg L-1 BAP (benzylaminopurine) + 1 mg L-1 ANA (naphthaleneacetic acid). In the multiplication phase, the shoots were sown on ulture media (MC1 = MS + 2.5 mg L-1 BAP + 1 mg L-1 ANA) and (MC2 = MS + 5 mg L-1 AP + 1 mg L-1 ANA). It was determined that the combination of a first and second disinfection with 8 and 4 % NaClO disinfected 80 % in Gran enano and 40 % in Hartón, respectively. In the multiplication phase, it was determined that the MS culture medium supplemented with 5 mg L-1 of BAP favored the formation of 8 banana shoots in both varieties.

Keywords : corms or tillers; explant; micropropagation; Musa spp.; phenolic oxidation..

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