Resumo

CCALTA HANCCO, Ruth; ORDONEZ-RODRIGUEZ, Cesar Domingo; CCALTA HANCCO, Ada Luz  e  CUCHO DOLMOS, Hernán Carlos. Cholesterol-loaded metil-β- cyclodextrin in alpaca semen cryopreservation (Vicugna pacos) The methodological alternative. J.Selva Andina Anim. Sci. [online]. 2022, vol.9, n.2, pp.39-48.  Epub 01-Out-2022. ISSN 2311-3766.  https://doi.org/10.36610/j.jsaas.2022.090200039.

The objective of the study was to evaluate the effect of three levels (0, 1.5 and 3 mg) of cholesterol loaded with methyl-β-cyclodextrin (MCD) in the cryopreservation of alpaca semen. Six male alpacas of the Huacaya breed from 6 to 8 years old were used, three for the collection of semen by the vaginal aspiration method (PC), and from three the EPZ were recovered by deviation of the vas deferens (DCD), three collections were madeper animal. Volume, filance, total motility, concentration, EPZ live, sperm membrane integrity and acrosomal integrity were evaluated. A Computer-assisted semen analysis (ISAS®) was used in the microscopic evaluations. The samples were diluted with Tris base to a concentration of 15 million EPZ per mL. It was added papain to samples collected by PC, followed by papain inhibitor. MCD was added in both methods, the cooled and thawed microscopic parameters were evaluated, a process in which dimethylformamide was used as a cryoprotectant. Macroscopic variables were evaluated using descriptive statistics, microscopic variables (mobility, live sperm, membrane functionality, and acrosomal integrity) were analyzed with a factorial arrangement in a randomized design. For all microscopic variables, significant differences (P<0.05) were found between the cooled stage in relation to thawing in both methods. For the PC method, no significant differences (P> 0.05) were found for mobility and vitality between treatments. However, EPZ treated with 1.5 mg of MCD presented higher (P<0.05) membrane functionality and acrosome integrity. In sperm from DCD, no significant differences (P> 0.05) were found between treatments for membrane mobility and functionality, but there was a significant difference (P< 0.05) for vitality and acrosomal integrity when 1.5 mg MCD relative to 3 mg MCD. In conclusion, the EPZ treated with 1.5 mg of MCD have better microscopic characteristics in cooled and thawed semen.

Palavras-chave : Alpaca; freezing; sperm; methyl-β- cyclodextrin; plasma membrane.

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