Abstract

CHOQUEHUANCA, José Luis; SOSA, Luis Fernando  and  BONIFAZ, Diego. Standardization and validation of elisa indirect type tests for the determination of the levels of IgG and IgE antileishmania antibodies, as a complementary method for diagnosis and follow-up of the response to treatmen. Rev.Cs.Farm. y Bioq [online]. 2019, vol.7, n.2, pp.39-54. ISSN 2310-0265.

Abstract Cutaneous leishmaniasis is character-ized by the induction of a cellular and humoral immune responses, at levels of IgG and IgE indicators serological diagnosis, progression and resolution of disease. In this study two indirect ELISA tests were stan-dardized to determine serum levels of IgG or IgE antibodies against soluble Leishmania braziliensis antigen (ASL). We worked from the strain of L. braziliensis (MHOM / BR / 75 / M2903). In the IgE ELISA pretreatment serum was done with anti-human IgG and Polyethylene glycol (PEG) antibodies; The optimal dilution of the sample for both ELISA was 1/21, the conjugate dilution was for 1/500 and 1/100 IgG ELISA for IgE. For standardized ELISA IgG index Kappa correlation of 1 (p <0.05) compared to commercial ELISA r-Biopharm® found.With a sensitivity of 100%; 100% specificity, 100% positive predictive value and negative pre-dictive value 100%. However both ELISA test showed cross-reactions anti-trypanosome antibodies. The results demonstrate that both ELISA can be used as diagnostic and monitoring tool. Being necessary to continue studies to validate these procedures and identify other possible cross-reactions.

Keywords : Leishmaniasis; ELISA; IgG; IgE; Standaritation.

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