Abstract

PALMA TOVAR, Camila Isabel et al. Application of the biochemical-colorimetric method to identify trypanocidal agents. Rev.Cs.Farm. y Bioq [online]. 2019, vol.7, n.2, pp.29-38. ISSN 2310-0265.

Abstract American trypanosomiasis is listed among the unattended infectious disease, is caused by the protozoan parasite Trypanosoma cruzi, and has no treatment in the chronic phase of this deadly disease. One of the challenges is finding effective therapies for this complex disease, given that it does not present any associated symptoms to the parasitism and is unknown among tradition-al doctors. Our Faculty is evaluating tacana traditional medicine as a source of potential antiparasitic agents. The objective of this work was to identify trypanocidal natural products using the XTT-PMS colorimetric method. For this, growth curves of T. cruzi epimastigotes were made to determine the optimal time to carry out the tests. The initial work population (3x10⁶ par-asites / mL), the incubation conditions (medium LIT, 27°C, 72 hours) and revealed process (XTT-PMS, 4 hours) were selected. With the optimized protocol, activity evaluations of control drugs, natural controls and 20 crude extracts of medicinal plants of the Amazon were carried out. The activity was based on calculations of mean inhibitory concentra-tion and substances with IC₅₀ < 50 μg/mL were considered active. Of the 20 extracts evalu-ated, 40% were active. The most interesting plants were Sipu sipu (IC₅₀= 8.9 ± 1.7 μg/mL), Ejije bid'u (IC₅₀= 9.1 ± 1.5 μg/mL) and Id'ene eidhue (IC₅₀= 10.8 ± 1.1 μg/mL) with values of IC₅₀ close to the controls, confirming the use-fulness and potential of the developed protocol.

Keywords : Trypanosoma cruzi; Tacana Culture; XTT-PMS Colorimetric Method; Medium Inhibitory Concentration (IC₅₀).

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