Resumen

TRIGO ORSINI, MYRIAM LINA. Validation of the method for the quantification of blood alcohol by gaseous chromatography with flame ionization detector and head and correlation space with the enzymatic method. Rev.Cs.Farm. y Bioq [online]. 2017, vol.5, n.2, pp.29-42. ISSN 2310-0265.

The presentstudy describes the standardization of the method for the quantification of alcohol in blood by Gas Chromatography with Flame Ionization Detector and headspace.The separation of the alcohol from a complex matrix such as blood was successfully carried out using an ELITE-1 column, the mobile phase or entrainmentgas is a mixture of hydrogen and air, split injector and flame ionization detector. The method was validated with the following parameters: specificity, linearity, precision, accuracy, limit of detection and limit of quantification. The system fitness test was also performed. The method was alcohol specific, the response was linear in the range of 0.125 -1.0 mg / mL analyte concentration. The value of the coefficient of variation or relative standard deviation (C.V. or DSR) for precision was optimal. The mean recovery was 99.06%. And the limit of detection and quantification were optimal for the quantification of blood alcohol in the defined range. Finally the chromatographic method was correlated with the enzymatic method (previously validated) currently used in the Ethical Dosage Division of the Institute of Technical Investigations of the "Mariscal Antonio José de Sucre" Police University, through the statistical analysis of the analytical responses of both methods a very good correlation was obtained by which it is concluded that the chromatographic method can be used for the consequent purposes.

Palabras clave : Validation; ethanol; gas chromatography; chromatographic method; enzymatic method.

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