Resumo

JURADO, M et al. Determination of the degree of infestation and infection of vectors of Chagas disease in the community of Huayhuasi (Mecapaca)  . Rev.Cs.Farm. y Bioq [online]. 2017, vol.5, n.1, pp.67-76. ISSN 2310-0265.

Abstract Trapping and capture of triatomines (which are the insect's vectors of Chagas' disease) were carried out in wild areas around the town of Huayhuasi, municipality of Mecapaca, in the department of La Paz. 50 traps were placed in strategic places chosen according to the characteristics already established. Of the 50 traps, nine traps managed to trap the triatomines, representing 4.5% of infestation in the study area. 13 insects (7 nymphs of different stages and 6 adults) were captured. The morphological characteristics of the insects were typical of Triatoma infestans. In the fresh examination of the excrement of the six captured adult specimens, in 4 of them the presence of the parasite Trypanosoma cruzi was detected, which shows a 67% infection, a very significant percentage considering the proximity of these infested places to the Urban areas of the municipality of Mecapaca. The parasites that the specimens carried were isolated and cultured in LIT (Liver Infusion Triptose) medium supplemented with 10% fetal bovine serum and incu-bated in an oven at26 ^°C.Analysis of the DNA extracted from the parasites has revealed the DTU corresponding to the Tc I lineage.

Palavras-chave : Triatoma infestans; DTU (Discrete Typing Unit); Reinfestation; Epidemiological risk.

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