Abstract

ENDALAMAW, Gadisa et al. Susceptibility studies on Leishmania aethiopica strains against total alkaloids from Galipea longiflora (Evanta). Rev.Cs.Farm. y Bioq [online]. 2016, vol.4, n.2, pp.11-19. ISSN 2310-0265.

Abstract The Instituto de Investigaciones Fármaco Bioquímicas (IIFB), at the Faculty of Pharmaceutical and Biochemical Sciences, from UMSA, carry out work related to the leishmanicidal activity of the total alkaloids (CAT) obtained from the bark of the Ama-zonian medicinal species known as Evanta (Galipea longiflora) by the Tacana, Tsimane y Mosetene people. As part of the activities develop by the UMSA-ASDI Project "Biomolecules of medicinal and industrial Interest (antiparasitic)" we had a visit, in our laboratories at IIFB, of a researcher from The Armauer Hansen Research Institute (AHRI) from Ethiopia, dur-ing his stay we were able to carry out evalu-ations of CAT, Miltefocine and Anphotericin B, against strains of L. aethiopica, causative agent of the different manifestations of cu-taneous leishmaniasis in Ethiopia. A total of six strains of L. aethiopica, were adapted to in vitro a conditions, at IIFB; and did show homogenous behavior against CAT. Five of the strains, showed an average calculated value for IC₅₀ = 8.68 ± 1.56 mg/mL, a value somewhat lower to the calculated for the reference strains L. ama-zonensis and L. braziliensis with IC₅₀ = 11.73 ± 4.32 mg/mL and IC₅₀ = 12.28 +/- 2.95 mg/ mL, respectively. Except for one strain that showed values somewhat higher, to the other strains, consistently through our studies, with IC₅₀ = 14.37 ± 3.58 mg/mL. As a consequence of our scientific inter-action, the Universidad Mayor de San Andrés (UMSA) has signed a Memorandum of Understanding for the development of joint research with the Armauer Hansen Research Institute (AHRI) that belongs to the Ministry of Health in Ethiopia, and explore the possi-bilities to replicate the Bolivian clinical val-idation experience of Evanta in the treat-ment of cutaneous leishmaniasis, in Ethiopia where the annual incidence is estimated to be between 20, 000 to 30, 0000.

Keywords : Bolivia; Etiopia; L. aetiopica; L.amazonensis; L.braziliensis; susceptibilidad Alcaloides; Galipea longiflora.

        · abstract in Spanish     · text in English     · English ( pdf )