Abstract

PARISACA MAMANI, SANDRA; BAUTISTA, ANGELA  and  VASQUEZ MICHEL, ANETH. Comparación del rendimiento del medio de cultivo Löwenstein-Jensen in house y Löwenstein-Jensen comercial, para el aislamientos de Mycobacterium tuberculosis de pacientes con tuberculosis pulmonar. Rev.Cs.Farm. y Bioq [online]. 2015, vol.3, n.1, pp.69-76. ISSN 2310-0265.

Tuberculosis (TB) has become a global health problem. In 2011 there were 8.7 million new cases. For TB diagnostic are used methods staining and solid media cultures: Löwenstein-Jensen (LJ), Ogawa, Stonebrink. The LJ is the reference media culture for evaluation of new systems for the diagnosis of TB. This study evaluated the performance of the commercial LJ culture media and LJ standardized by the National Tuberculosis Laboratory Network. 185 sputum samples BK(+), being plated in duplicate in the LJ in house medium and commercial LJ medium were processed. By in house LJ medium were obtainned, 172 positive cultures (93%), 9 (5%) negative and 4 (2%) contaminated; through commercial LJ were obtained 148 positive (80%), 8 negative (4%) and 29 (16%) contaminated. In in house LJ medium an eugónic development was obtained in 131 (76%) and disgónic development in 41 (24%) cultures; in commercial LJ we saweugonic development in 29 (20%) and disgonic development in 119 (80%) cultures. When comparing between in house LJ and commercial LJ a kappa value of 0.96 was obtained, demonstrating astrength of concordance of 0.96 (almost perfect). The results show that in house LJ medium (standardized by the National Tuberculosis Laboratory Network) surpass commercial LJ, as show better development and a lower percentage of contamination, thereby facilitating further drug resistance studies.

Keywords : Löwenstein-Jensen; Tuberculosis; National Tuberculosis Laboratory Network.

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