Abstract

SALAMANCA CAPUSIRI, EFRAIN¹ et al. Susceptibilidad in vitro de promastigotes de Leishmania frente a Anfotericina B, Miltefosina y los Alcaloides Totales de Galipea longiflora mediante el Método Colorimétrico XTT-PMS. Rev.Cs.Farm. y Bioq [online]. 2015, vol.3, n.1, pp.29-36. ISSN 2310-0265.

This paper presents research results in determining the in-vitro susceptibility of 27 Leishmania promastigote cultures adapted to in-vitro conditions from skin lesion sam-ples of patients lending assistance to IN LASA laboratories. Antiparasitic activity was measured in the following: Amphotericin B, Miltephosine, and the total alkaloids pres-ent in Galipea longiflora. Results using IC50 show that the various isolated strains of L.m.amazonensis and L.b.braziliensis show a wide spectrum of susceptibility to Amphotericin B, from 0.06-0.42ug/mL to 0.03-0.41 ug/mL; to Miltephosine, from 1.67-12.93ug/mL to 1.25-21.17ug/mL; and to the total alkaloids of Galipea longiflora, from 14.4-39.55ug/mL to 6-22.05ug/mL respec-tively. Application of the XTT-PMS colorim-etry method reveals strains of Leishmania in circulation with a wide spectrum of in-vitro susceptibility. L.b.braziliensis showed a wide IC50 dispersal with Amphotericin B, up to 14 times greater than the most sen-sitive strain; Miltephosine showed an IC50 dispersal up to 17 times greater than the most sensitive strain; whereas the alkaloids of Galipea longiflora showed an IC50 dispersal of only 3 times greater than the most sensitive strain. This dispersal data is con-gruent with previous research regarding the treatment of Leishmaniosis in Bolivia. Colo-rimetry techniques are an important tool in determining susceptibility to antiparasitics used in Bolivia.

Keywords : Susceptibility; antiparasitic; XTT-PMS; Amphotericin B; Miltephosine; Galipea longiflora; L.m.amazonensis; L.b.braziliensis; strains in circulation; Leishmania; Leishmaniosis; Bolivia.

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