Resumo

CONDORI S., RAMIRO; ROJAS H., RICARDO; GUTIERREZ A., ROXANA J.  e  PINO-FIGUEROA, ALEJANDRO. Evaluación in-vitro del efecto de la curcumina y curcumina dietil succinato sobre la viabilidad y producción de il-6 en cultivos celulares de cáncer de mama. Rev.Cs.Farm. y Bioq [online]. 2015, vol.3, n.1, pp.17-26. ISSN 2310-0265.

Curcumin is a polyphenol used as natural dye obtained from Turmeric (Curcuma Longa) which is native from India. Different studies have identified the curcumin as the most active metabolite of turmeric (Curcuma Longa); in-vitro studies demonstrate anti-inflammatory, anti-oxidant and anti-cancer activity of curcumin. This research was carried out in the Laboratory of Farmacology from the Massachusetts College of Pharmacy and Health Sciences University, Boston, USA in the period from May to August in order to assess the activity of curcumin and curcumin diethyl succinate (analogous synthetic compound of curcumin with increased stability) over the cellular viability and one of the mechanisms of proliferation and metastasis of breast cancer, which is the IL-6 production. We used cell cultures of breast cancer (MDA-MB 231 cell line) treated with curcumin and curcumin diethyl succinate in different concentrations: 1 uM, 3 uM and 10 uM in both cases. After incubation of 24 hours, it was determined the cellular viability with tripan blue and MTS assay, the concentration of IL-6 was determined by ELISA (Enzyme Linked Assay by immunoabsorption). Our results showed a decrease in the proliferation of cancer cells (viability) with both curcumins compared with the control group, being the viability of the cultures treated with curcumin lower than the cells with diethyl succinate curcumin. In relation to the IL-6 production, itwas confirmed that cancer cells produce very high concentrations of this cytokine (90-98 pg / ml, values corresponding to the controls used), which were visibly reduced after cell incubation with curcumin and diethyl succinate curcumin at concentrations of 1, 3 and 10 uM. Also, itwasshown statistically that curcumin produced a greater reduction in IL-6 levels than the diethyl succinate curcumin in concentrations of 3 and 10 uM, however at 1 uM the synthetic curcumin was more efficient. When we relate the reduc-tion of cell viability caused by both curcumins, with the production of IL-6, itwas observed that there is not a direct relationship between such parameters because even with the significant decrease in cell proliferation induced by curcumins, IL-6 production in cell cultures remained higher than the values observed in healthy cells. Itwas concluded that curcumins modify cell morphology of cancer cells, reducing their viability and IL-6 production, nothaving found a direct relationship between these two variables.

Palavras-chave : IL-6 (interleucina 6); viabilidad celular; curcumina; curcumina dietil succinato; células de cáncer de mama.

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