Resumen

CHOQUE, CRISTIAN. Desarrollo de un metodo analitico indicador de estabilidad y biodisponibilidad en vitro por hplc para microencapsulado de acido acetilsalicilico y cafeina. Rev.Cs.Farm. y Bioq [online]. 2014, vol.2, n.1, pp.103-115. ISSN 2310-0265.

Is developing a stability indicator and biodisponobilidad analytical method by HPLC ( High Resolution Liquid Chromatography ) for the determination of acetylsalicylic acid and its degradation product Salicylic Acid plus the determination of caffeine in a solid dosage form in this case a granules . To establish the validity of the analytical method parameters specificity, linearity and range, precision, accuracy, limit of quantification and limit of detection using a liquid chromatograph Agilent High Resolution HPLC, column chromatography with silicabased chemically bonded were determined a hydrocarbon chain of 18 carbon atoms, L1 according to USP ( United States Pharmacopeia ) a mobile phase composed of 85% monobasic sodium phosphate adjusted to a concentration of 25 mM at pH 2.4 and 15% HPLC grade acetonitrile, this method was used for the quantification of acetylsalicylic acid and Caffeine granules containing mannitol, alcohol, FD & C Yellow No. 6, saccharin, flavor orange spray, sodium bicarbonate, citric acid, copovidone, copolymer of meta acrylic acid as an agent for enteric coating. Bioavailability vitro micro encapsulated aspirin with enteric coating agent and in addition to caffeine which was evident that the analytical method is the ability to quantify these active ingredients in a first step subjected two hours gastric fluid was then determined simulated and from there an hour in simulated intestinal fluid in vitro dissolution equipment Pharma Test

Palabras clave : validation; bioavailability; stability; HPLC.

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