Abstract

QUISPE, SERGIO; TORRICO, BERNARDO  and  GUEVARA, PALMIRA. Diagnóstico Molecular de la Enfermedad de Chagas mediante Amplificación Isotérmica mediada por asas (LAMP). Rev.Cs.Farm. y Bioq [online]. 2014, vol.2, n.1, pp.17-24. ISSN 2310-0265.

The monitoring of triatomino infections is a key step in the epidemiology of Chagas disease. We demonstrate the feasibility of incorporating new molecular identification tools, we obtained positive LAMP identification of infections by T. cruzi and T. rangeli in haemoliph and digestive track samples preserved in filter paper. Loop-mediated isothermal amplification (LAMP) is a novel nucleic acid amplification method thatamplifies DNA with high specificity, efficiency and rapidity under isothermal conditions using a set of four specially designed primers and a DNA polymerase with strand displacement activity. We have applied a method that accelerates the LAMP reaction by using additional primers, termed loop primers. The LAMP method presented here uses loop primers to achieve reaction times of less than half that of the original LAMP method, this reaction was applied to samples from Tripanosoma rangely and Tripanosoma cruzy. Since the total time of analysis including detection is less than 1 h, this new method should facilitate genetic analysis, including genetic diagnosis in the clinical laboratory

Keywords : LAMP; isoterm; DNA.

        · abstract in Spanish     · text in Spanish     · Spanish ( pdf )