Abstract

PEREZ-GUZMAN, Jheanete  and  AGUILAR-TINTAYA, Melisa Lidia. Evaluation of the multiplication of three native potato (Solanum sp.) varieties for the conservation of native species. J. Selva Andina Biosph. [online]. 2023, vol.11, n.1, pp.66-75.  Epub May 01, 2023. ISSN 2308-3867.  https://doi.org/10.36610/j.jsab.2023.110100063.

Unfortunately, in Bolivia native varieties are being displaced by commercial ones, causing the reduction or discarding of their production because they do not have a secure market, putting their conservation at risk. The objective of this research is to evaluate two M & S culture media in the multiplication phase of three native potato varieties (Solanum sp.) from the Millimbaya community, Tacacoma municipality, for the conservation of native species. Therefore, three native potato varieties from Tacacoma were sampled: Pichuya, Llok´alla y Pato Kayo, which, after a process of selection of explants and disinfection of shoots, were established in vitro at 21±1° C, 2000 lm light flux and photoperiod 16:8 h, for 25 days. In the multiplication phase, the asymmetric factorial experimental design was used for the study of the factors: variety and culture medium, where the evaluated response variables were: stem height, number of leaves and internodes. The results of the research showed the preference of the varieties Pichuya and Llok´alla to the MS1(MSP01) medium composed of 332.2 mg/L CaCl2, 180.7 mg/L MgSO4 and 16.9 mg/L MnSO4, when presenting greater averages in length, number of leaves and internodes than when cultivated in MS2 medium, and the favorable response of the Llok'alla variety to MS2 medium.

Keywords : Genetic erosion; multiplication; culture medium; Murashige and Skoog; native varieties; in vitro; conservation.

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