Abstract

PEREZ-GUZMAN, Jheanete  and  QUENTA-HERRERA, Estefanía. Germination in vitro embryo of Walnut (Juglans boliviana). J. Selva Andina Biosph. [online]. 2015, vol.3, n.1, pp.15-23. ISSN 2308-3867.

Bolivian Juglans is an important forest species found in the rain forests of Bolivia. The seed of this species is recalcitrant with hardened cover, which hinders germination and propagation of the species. The aim of this study was to determine the culture medium for in vitro germination of mature embryos of Bolivian Juglans. Technique initially scarification and disinfection process was determined. Subsequently in vitro culture was performed using the culture medium Woody Plant Medium (WPM) with the addition of plant growth regulators (indole butyric acid and 6-benzyl aminopurine) in different concentrations. As control WPM, culture medium was used 100%. Response variables evaluated were percentage of contamination and germination; vitroplant length, number of leaves, number of shoots, number of roots per vitroplant, root length and percentage of survival. The plantlets in vitro germination in treatments and the control in the middle l culture WPM supplemented with 0.15 mg / l of IBA and 1.5 mg / l BAP was 90%; other treatments inhibit the growth of the stem and roots of plantlets.

Keywords : Germination; seed; embryo; scarification; medium of cultivation; phytoregulators.

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