Abstract

DE LA CRUZ, María et al. Effect of oligosaccharides and enzymes on biofilms generated by Candida glabrata in dental prostheses. Rev. Cien. Tec. In. [online]. 2019, vol.17, n.20, pp.67-82. ISSN 2225-8787.

Abstract C. glabrata frequently colonizes the oral cavity of older adults. Biofilms generated by C. glabrata with a diminished sensitivity profile or resistance to fluconazole attached to dental prostheses, are a risk factor for subprothetic candidiasis and mortality in hospitalized older adults. The resistance of C. glabrata, especially for its ability to produce biofilms, leads to dental therapeutic failure and requires researching therapeutic alternatives. Therefore, the aim of this work was to study the effect of oligosaccharides as antifungals and hydrolytic enzymes as biomolecules reducing biofilms generated by C. glabrata. 37 strains of C. glabrata (11 of them, biofilm-forming) isolated from 81 older adults (with informed consent) residing in care homes in Sucre, Bolivia, were analyzed. The antifungal susceptibility of oligosaccharides was studied by diffusion in agar and MIC by means of microdilution in RMPI broth according to CLSI. The activity on biofilms according to the O'Toole and Kolter method. Results: Ch-D chitosan showed good antifungal activity for C. glabrata. The use of lysozyme at 50 ug / ml shows an excellent activity as a 24-hour biofilm reducing agent in dental prosthesis isolates. Conclusions: Our study showed that chain length, such as the presence of amino groups in oligosaccharides, are key to antifungal activity against C. glabrata. In the case of biofilms formed, the activity of lizosima could be useful. Both molecules could be a good alternative for the treatment of C. glabrata and avoid the appearance of subprosthetic candidiasis.

Keywords : Candida glabrata; biofilms; oligosaccharides; lysozyme; chitosan.

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