Abstract

PEREZ-DELGADO, Orlando; ALVARADO-PINEDA, Rosa Liliana  and  YACARINI-MARTINEZ, Antero Enrique. In vitro antibacterial activity of crude ethanolic extract from the leaves of Origanum vulgare, against Staphylococcus aureus ATCC 29213, Pseudomonas aeruginosa ATCC 27853 and Escherichia coli ATCC 25922. J. Selva Andina Res. Soc. [online]. 2021, vol.12, n.1, pp.21-29. ISSN 2072-9294.

The present work whose objective was to evaluate the in vitro antibacterial activity of the ethanolic extract of Origanum vulgare leaves against Staphylococcus aureus ATCC 29213, Pseudomonas aeruginosa ATCC 27853 and Escherichia coli ATCC 25922. The study was experimental, 72 experimental units were used, consisting of a type of ethanolic extract three concentrations, three bacterial species and eight repetitions per experimental group. Through the serial double dilution method, the different concentrations were determined, for the antibacterial activity, the well diffusion method was used. Concentrations of 80 mg/mL, 40 mg/mL and 20 mg/mL were used. The ethanolic extract showed antibacterial activity in vitro, with an average size of the inhibition halos for S. aureus of 21.64, 15.24 and 11.45 mm, P. aeruginosa 13.31, 12.27 and 7.35 mm, E. coli 12.5, 11.40 and 10.6 mm for the different concentrations. It is concluded that the ethanolic extract of O. vulgare has antibacterial capacity on S. aureus ATCC 29213, P. aeruginosa ATCC 27853 and E. coli ATCC 25922.

Keywords : Origanum vulgare; Staphylococcus aureus; Pseudomonas aeruginosa; Escherichia coli; antibacterial; ethanolic extract.

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