Abstract

SAUCEDO-MARTINEZ, Blanca Celeste; FARIAS-RODRIGUEZ, Rodolfo; SANTOYO-PIZANO, Gustavo  and  SANCHEZ-YANEZ, Juan Manuel. Integral biostimulation of soil polluted by 60000 ppm of motor waste oil. J. Selva Andina Res. Soc. [online]. 2017, vol.8, n.2, pp.91-103. ISSN 2072-9294.

Waste motor oil (WMO) is a mixture of hydrocarbons (HICO) soil pollutants. An alternative solution for its elimination is the biostimulation (BIS), secuancial, complementary and accumulative or integral which requires at first BIS by detergents to emulsify WMO, the second one following by enrichment by mineral solution, H₂O₂  as a O₂ source for oxidation of WMO and controlling moisture soil content at 80% field capacity for exchange gases in soil to stimulate WMO mineralization  The aim of the work was: i) analyze in the integral BIS of contaminated soil by 60000 ppm of WMO. The first BIS was applying detergents to emulsify the WMO, the second BIS by mineral solution, then by H₂O₂, under humidity control at 80% of field capacity, for the best oxidation of WMO. Additionally bacterial population oxidant WMO was measured, to select those which synthetized detergent related to WMO degradation, finally the type of bacteria were molecular identify. Results showed than integral BIS by an anionic / nonionic detergent, then enrichment with mineral solution, and 0.5% H₂O₂, reduced WMO from 60000 ppm to 27200 ppm in 23 days. Density of bacterial WMO oxidant population was 268 X 10⁶ CFU/g dry soil, from which the dominant ones were selected and identified 47 bacterial genera divided into: Actinomycetes, Firmicutes and Proteobacteria. These results showed that soil pollution by high concentration level of WMO demanded at integrated BIS, also was found some bacterial genus which synthetized detergent with potential used in soil polluted by WMO.

Keywords : Soil; WMO; detergent; mineral solution; H₂O₂; Mineralization.

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