Abstract

ANCASI-ESPEJO, Ruth Gabriela; MONTERO-TONCONI, Julio Ricardo; FERREIRA-CASTEDO, Napoleón Juan  and  MUNOZ-GUZMAN, Israel. Determination better culture medium in the establishment phase for the in vitro propagation of banana (Musa paradisiaca L). J. Selva Andina Res. Soc. [online]. 2016, vol.7, n.2, pp.104-111. ISSN 2072-9294.

This research was conducted at the Laboratory of Plant Biotechnology of the Department of Biological and Natural Sciences of the Amazonian University of Pando, in 2014. The aim of the study was to determine better culture medium in the establishment phase for propagation in vitro banana (Musa paradisiaca L.), 20 were selected and characterized mother plants NTRCA (New Technology Research Center Amazonia). A completely random design (CRD) with three different culture media was used. The culture media were M1) Murashige and Skoog (MS) was supplemented with ascorbic acid 100 mg/L and L-cysteine 2 ml /L, M2) Murashige and Skoog (MS) was supplemented charcoal 2 g/L, M3) Murashige and Skoog (MS) supplemented with ascorbic acid 100 mg/L and cítrico100 mg/L acid. The variables evaluated were: The survival of the former Plantes, where contamination and oxidation was observed. The results showed that in the first phase of establishment, the best answer for the survival of the former Plantes banana (Musa paradisiaca), was with the culture medium 3, where a lower degree of oxidation (0.26) and pollution for all explants was obtained was 28%.

Keywords : In vitro culture; propagation; establishment; contamination; disinfection.

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