Abstract

ZOTTA, Claudio Marcelo; LAVAYEN, Silvina; NARIO, Flavia  and  PIQUIN, Andrea. Detection of Escherichia coli Shiga toxin-producing in viscera of animals bovine and chicken intended for human consumption. J. Selva Andina Res. Soc. [online]. 2016, vol.7, n.1, pp.2-9. ISSN 2072-9294.

Escherichia coli producing-Shiga toxin (STEC) is associated with foodborne illness (ETA). It can cause bloody diarrhea, hemorrhagic colitis, hemolytic uremic syndrome and thrombotic thrombocytopenic purpura. The aim of the study was to detect the presence of STEC in samples of organs (offal) of bovine animals and chicken intended for human consumption. Between 2008-2009, 76 samples  bovine entrails and 22 chicken viscera samples, were processed  and underwent, as screening technique, the polymerase chain reaction (PCR) for detection of multiple genes coding for the factors virulence: Shiga toxin (stx₁, stx₂) and rfb_O157 gene coding for capsular O157 lipopolysaccharide LPS. Samples from bovine offal development showed 84.2% for coliform bacteria. These isolates showed no virulence factor that characterized as STEC or Escherichia coli O157. The chicken offal samples showed 95.5% of development for coliform bacteria, being negative for the presence of genes encoding the Shiga toxins 1 and 2 (stx₁, stx₂) and rfb_O157 gene. While this work does not STEC was detected, the presence of coliform bacteria in the samples studied makes these foods should be considered as potentially hazardous to consume undercooked with the consequent possibility of filing ETA.

Keywords : Escherichia coli; producing-Shiga toxin; hemolytic uremic syndrome; serotype O157:H7; viscera; foodborne illness.

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