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Journal of the Selva Andina Research Society

On-line version ISSN 2072-9294


GARCIA-ORTIZ, Vanessa Ruby; MARQUEZ-BENAVIDEZ, Liliana  and  SANCHEZ-YANEZ, Juan Manuel. Evaluation of the phytotoxicity of Landfill leachate on Phaseolus vulgaris L and survival of microorganisms of public health importance. J. Selva Andina Res. Soc. [online]. 2015, vol.6, n.2, pp.51-63. ISSN 2072-9294.

In landfill, the organic fraction of municipal solid waste generated leachate (LE), a chemical inorganic and organic mix an environmental pollutant to determine by Leachate Pollution Index (LPI). A bioassay could give certainty to its negative effect. To include pathogenic human microorganisms (PHM) and mesophilic total aerobic bacteria (MTAB) different than fecal and total coliforms would have health value. The objectives of this research were i) analyzed the phytotoxicity of leachate on Phaseolus vulgaris and ii) to detect in LE the existence of HPM and MATB. A bioassay "in vitro" was made in Petri dishes and Leonard jars using seeds P. vulgaris treated by diluted leachate. The response variables of bioassay were: germination percentage, phenotype: plant height and root length, biomass total: fresh and dry weight of root and plant. Including HPM and MATB detection. Experimental data was analyzed by Tukey p<0.05. The results indicated that LE caused inhibition and/or stimulation on P. vulgaris; Leachate´s phytoxicity was depending on its chemical composition and level dilution, this suggests that toxic effect is due its heavy metals and extreme alkalinity, killing HPM in leachate but not MATB. A bioassay P. vulgaris is useful to define degree of benefical and/or toxicity of LE, and validate LPI. While is important to know the role of HPM in LE and potential environmental biological contamination.

Keywords : Leacheate; plant bioassay; alkalinity; microbial survival.

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