Resumo

BONIFAZ PEREZ, Diego  e  ROCABADO CALIZAYA, Omar. MOLECULAR IDENTIFICATION OF ONCOGENIC HPVS BY REAL TIME PCR WITH TAQMAN PROBES. Rev Cient Cienc Méd [online]. 2020, vol.23, n.2, pp.122-128. ISSN 1817-7433.

Introduction: Human Papillomavirus (HPV) constitute a diverse group of viruses, being genotypes 16 and 18 the most prevalent and main objectives of several studies on anogenital tract infections. The present study intends to determine the High Risk HPVinfection in genital samples,through the application of molecular technology for the genotyping of HPV16 and HPV18 by Real Time PCR. Materials and methods: We worked with 151 samples of genital swabs where DNA extractions were performed using silica columns and the identification of High Risk HPV was through the optimization of a duplex real-time PCR for genotyping of HPV 16 and 18, for which primers and TaqMan® probes were designed by specialized software, and ideal reagent concentrations and reaction temperatures were determined. Results: In the identification of HPV High Risk , a total of 41 positive cases were obtained. These samples were selected to perform genotyping of HPV16 and HPV18 using the Real Time PCR technique. The greater presence of these oncogenic viruses was determined in women aged 15 to 29 years (40% and 26.7% respectively). Conclusion: Through the optimization and implementation of new molecular techniques, it is intended to improve the epidemiological follow-up on the presence of High Risk HPV and thus provide a better guide on the dissemination and presence of the different oncogenic HPV genotypes in our population.

Palavras-chave : Papillomaviridae; Real Time PCR; Cervical Cancer.

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