Abstract

CASABLANCA ALARCON, Esther; RIOS MANRIQUEZ, Neida; TERESA ALVAREZ, María  and  TERRAZAS SILES, Enriquen. Optimization of anaerobic thermophilic culture conditions for increasing xylanase enzyme production with encapsulated and free bacteria strains. BIOFARBO [online]. 2009, vol.17, n.2, pp. 51-59. ISSN 1813-5363.

The thermophilic bacteria that produce enzymes have a big interest in biotechnology, due diverse application in the industry for extreme stability. These biocatalysts are used in the preparation of pulp paper, food processing agroindustrial and hemicellulose conversion to biomass. The purpose of this work is to increase the enzymatic production of the xylanases of thermophilic anaerobic bacteria produced by three strains FT3, 2B and P5 which were isolated from the Bolivian plateau. The strain FT3 was selected with significant xilanolitic activity of 2.0 IU / mL compared with the other strains. The optimization of culture medium was performed in free and encapsulated cell, changing the culture medium concerning nitrogen source, carbon source and pH. The best nitrogen source went yeast extract 2.46 IU / mL with bacteria-free and 10.1 IU/mL in bacteria-encapsulated and best carbon source is the soybean straw (Glycine max) as hydrolyzed material and coming up as raw material to get up to 2.2 IU / mL and 1.9 IU / mL respectively at pH 8.

Keywords : xylanases; immobilization.