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Cuadernos Hospital de Clínicas
Print version ISSN 1562-6776
Abstract
CHAVEZ RAMOS, MA et al. ELABORATION AND STANDARDIZATION OF THE ALSEVER SOLUTION FOR THE PRESERVATION OF HUMAN ERYTHROCYTES UNDER ALTITUDE CONDITIONS IN LA PAZ, BOLIVIA. Cuad. - Hosp. Clín. [online]. 2025, vol.66, n.1, pp.19-30. Epub Sep 23, 2025. ISSN 1562-6776.
In the immunohematology laboratory, red blood cell preparation is crucial to validate various blood group phenotyping procedures. Preservation of red cells in physiological solution can reduce ATP by 80-90%1, leading to morphological changes and increased osmotic fragility. Alsever solution, an anticoagulant and isotonic saline preservative, is used in various disciplines, but due to its high cost and lack of availability in the country, it is necessary to prepare and standardize it in local laboratories.
OBJECTIVES: To prepare and evaluate the effectiveness of modified Alsever solution in the preservation of human erythrocytes of different blood groups under altitude conditions in La Paz, Bolivia.
MATERIALS AND METHODS: A case series study was conducted with 10 random blood donors with blood types A, B, and O, with a control group.
Erythrocyte survival was evaluated in two groups: one in modified Alsever solution and the other in 0.9% physiological solution. Panels were prepared at a concentration of 3% erythrocytes in both solutions. Physicochemical, hematological, hemolysis, hemagglutination and pH tests were carried out using automated equipment and the results analyzed at different storage times.
RESULTS AND CONCLUSIONS: The results showed that the modified Alsever solution preserves erythrocytes for up to 42 days, exceeding the preservation time of physiological solution. In addition, a protocol for the preparation of erythrocyte cells of known phenotype was established, which improves hemagglutination procedures. This study supports the effectiveness of the modified Alsever solution in high altitude regions.
Keywords : Immunohematology; Alsever; phenotype; agglutination; high altitude.
