Abstract

AMARU, Ricardo et al. DNA-UMSAgen, EXTRACCIÓN DE DNA GENÓMICO PARA DIAGNÓSTICO MOLECULAR: MÉTODO RÁPIDO Y ECONÓMICO. Cuad. - Hosp. Clín. [online]. 2006, vol.51, n.2, pp.11-15. ISSN 1562-6776.

INTRODUCTION DNA is the genetic material of the cell. Actually for its study, laboratory techniques are available that require its extraction free of impurities. This paper describes the DNA-UMSAgen method as an alternative for DNA extraction which is based on the Miller technique. METHODS The mononuclear cells were obtained of venous peripheral blood of a voluntary subject, for to realize 40 DNA's extractions; 20 with the modified method DNA-UMSAgen and other 20 with the classic method. Later there was evaluated the concentration, quality and utility of these extracted DNA. RESULTS The DNA purity extracted by the DNA-UMSAgen method is 1,88 similar to the classic technique 1,91, the concentration obtained were 20,4 ug/106 cells and 56ug/106 cells respectively. The evaluation by agarose gel electrophoresis and the amplification of the exon 12 of the JAK2 gen by PCR was successful. CONCLUSION The DNA-UMSAgen extraction method is a very acceptable fast, easy and inexpensive alternative method for underdeveloped countries for DNA extraction.

Keywords : DNA; extraction; spectrophotometry; PCR; agarose gel electrophoresis.

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