Abstract

ADAMCZYK WIELER, Lorena; TRIGOSO AGUDO, Christian; VASQUEZ MICHEL, Aneth  and  DURAN ARIAS, Loretta. Determinacion de la Homoresistencia y Heteroresistencia de Staphylococcus Aureus Meticilinoresistente aislado de muestras de pacientes internados en los hospitales Boliviano Holandes, Hospital Obrero, y Hospital de Clínicas. Cuad. - Hosp. Clín. [online]. 2005, vol.50, n.2, pp.7-11. ISSN 1562-6776.

Research Question Are the circulating methicillin resistant staphylococcus aureus strains in our patients homoresistant or heteroresistant? Objectives To assess the homoresistance and heteroresistance to methicillin of Staphylococcus aureus isolated from purulent exudate samples obtained from patients admitted to the Hospital Boliviano Holandes, Hospital Obrero and Hospital de Clínicas in La Paz. To determine the percentage of methicillin resistant Staphylococcus aureus in these samples. Study Design Cross sectional Study Location Instituto Nacional de Laboratorios de Salud (INLASA) Samples The strains were isolated from samples of purulent discharges from abscesses, wounds, infected burns from patients admitted to the Hospital Boliviano Holandes, Hospital Obrero, and Hospital de Clinicas. Methods Staphylococcus aureus was identified by tests of catalase, coagulase and manitol. Sensitivity and/or resistance was determined by disc diffusion (Bauer Kirby) with discs of Oxacilina of 1 μg . The Minimal Inhibitory Concentration (CIM) was determined by microdilution. Results Of 100 Staphylococcus aureus isolations, 16% were methicillin resistant. None of these strains was shown to be homoresistant. Therefore, we assume that 100% of the strains were heteroresistant. Conclusions Methicillin maintains a good antimicrobial activity against Staphylococcus aureus. The isolations in our study did not show homoresistance.

Keywords : Methicillin; Staphylococcus aureus; homoresistance; heteroresistance; disc diffusion; minimal inhibitory concentration.

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