Objective:

to evaluate the antibiofilm effectiveness of maqui extract (Aristotelia chilensis) on Escherichia coli under in vitro conditions in the year 2025.

Methods:

an experimental longitudinal in vitro study was conducted using the Escherichia coli ATCC 25922 strain. An extract of maqui leaves was prepared through maceration in water and ethanol. The minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) were determined using broth dilution methods and colony-forming unit (CFU) counting. The antibiofilm activity was assessed using the crystal violet staining method, measuring optical density.

Results:

the maqui extract exhibited a MIC of 10 µg/mL and an MBC of 100 µg/mL. At concentrations above 60 µg/mL, the inhibition of bacterial growth was comparable to tetracycline. Biofilm formation was significantly reduced at concentrations starting from 10 µg/mL, showing a growing inhibitory effect as the concentration of the extract increased.

Conclusions:

the results suggest that maqui extract has potential as a natural antimicrobial agent against Eschericha coli, particularly in inhibiting biofilm formation. This highlights the importance of exploring plant-based alternatives in the context of increasing antibiotic resistance, offering new avenues for the development of treatments based on natural compounds. Further in vivo studies are recommended to confirm the observed bioactivity.

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