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Gaceta Médica Boliviana
versão impressa ISSN 1012-2966versão On-line ISSN 2227-3662
Resumo
GRADOS-TORREZ, Ricardo Enrique et al. In silico analysis of the sequence and structure alterations of RBD-SARS-CoV-2, which affect its complementarity by IgG-antiRBD neutralizing antibodies. Gac Med Bol [online]. 2023, vol.46, n.2, pp.76-80. Epub 01-Dez-2023. ISSN 1012-2966. https://doi.org/10.47993/gmb.v46i2.664.
Anti-SARS-CoV-2 vaccines induce the production of IgG neutralizing antibodies against the Receptor Binding Domain of the S protein of the virus (IgG-antiRBD). In Bolivia, Sinopharm and Sputnik V were widely used vaccines during the pandemic. However, the mutations and changes suffered in SARS-CoV-2 were responsible for the new contagion outbreaks.
Objective:
To determine the sequence and structure alterations of RBD-SARS-CoV-2, which affect its complementarity by IgG-antiRBD neutralizing antibodies.
Material and Methods:
The sequences and crystallographic structures of RBD-SARS-CoV-2 were obtained from the Protein Data Bank database. For Multiple Sequence Alignment and Structural Alignment, Mega6 and Chimera1.15 were used.
Results:
The Multiple Sequence Alignment and Structural Alignment of the main epidemiological variants of SARS-CoV-2 showed that krakenXBB1.5 was the most divergent at the sequence level, while omicronBA2.75 presented more structural changes and greater steric hindrances when interacting with IgG-antiRBD, being the most contagious and most evasive to the immune response.
Conclusions:
The use of bioinformatics tools for monitoring the molecular changes of SARS-CoV-2 make it possible to predict the epidemiological behavior of new emerging variants and also promote improvement in prevention criteria
Palavras-chave : molecular biology; biotechnology; immunology; mutation; SARS-CoV-2.
