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Gaceta Médica Boliviana
versão impressa ISSN 1012-2966versão On-line ISSN 2227-3662
Resumo
SERRANO-SEGURA, Kevin et al. Evaluation of a nested multiplex polymerase chain reaction test for the detection of HTLV-1. Gac Med Bol [online]. 2023, vol.46, n.2, pp.32-36. Epub 01-Dez-2023. ISSN 1012-2966. https://doi.org/10.47993/gmb.v46i2.608.
Peru is an endemic area for human T-lymphotropic virus type 1 (HTLV-1) and for its diagnostic confirmation serological tests are used, which can give inconclusive results.
Objectives:
to evaluate a nested multiplex PCR test to diagnose HTLV-1.
Methods:
PCR validation was performed with primers targeting the Pol and LTR regions of HTLV-1. The β-globin gene was used as an internal endogenous control and the detection limit was evaluated with MT2 cells. Diagnostic accuracy parameters were evaluated against 95 Reference blood samples.
Results:
the evaluated test obtained a detection limit of 0.5 ng/μL of DNA; diagnostic sensitivity=97.1%, diagnostic and analytical specificity=100%, vpn=97.2%, vpp, repeatability and reproducibility=100%; Kappa, Youden Index=0.97.
Conclusions:
the evaluated test has a high diagnostic performance and due to its low cost, its implementation in the HTLV-1 diagnosis algorithm in Peru is recommended.
Palavras-chave : diagnosis; retrovirus; nested PCR; nested PCR nested PCR nested PCR.
