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Gaceta Médica Boliviana

On-line version ISSN 1012-2966


CORDOVA R, N. Marisol et al. Differential patterns between leishmaniasis and chagas disease using Trypanosoma cruzi epimastigotes . Gac Med Bol [online]. 2020, vol.43, n.2, pp.120-126. ISSN 1012-2966.

In different regions of Latin America, infection by T. cruzi and Leishmania overlap, for which mixed circulating infections are reported, due to this; Specific diagnostic tests must be performed to avoid cross reactions between these two pathologies. Objective: to determine fluorescence patterns that allow the differentiation between Leishmaniasis, Chagas disease and mixed infection using T. cruzi epimastigotes. Methods: the Indirect Immunofluorescence technique was used using epimastigotes of T. cruzi (autochthonous TcV) as figurative antigen against a panel of serum samples coded as A, B, C and D corresponding to patients with infection by: Leishmaniasis (A) , Mixed infection by Leishmania and Chagas (B), Chagas disease (C) and without either of the two infections (D). Results: in the four sample panels, different patterns of fluorescence intensity were observed at the membrane and nucleus level of the epimastigotes of T. cruzi (autochthonous TcV). Conclusions: the Immunofluorescence technique (IFI) with T. cruzi epimastigote antigens has proven useful in differentiating between Chagas disease, Leishmaniasis and / or mixed infections by both parasites in areas where the coexistence of both is common.

Keywords : leishmaniasis; chagas; Trypanosoma cruzi; epimastigotes; Fluorescent Antibody Technique Indirect.

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