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Gaceta Médica Boliviana

On-line version ISSN 1012-2966


CORDOVA ROJAS, Marisol; CRUZ TORRICO, Mary  and  TORRICO, Faustino. Acquisition mode pesticides and drugs in intoxicated patients treated in emergency Hospital Clínico Viedma. Gac Med Bol [online]. 2015, vol.38, n.1, pp.6-9. ISSN 1012-2966.

Objective: to evaluate the use of cell cultures for the production of trypomastigotes in 3T3 cells, from a local strain of Trypanosoma cruzi epimastigotes. Method: we previously performed growing 3T3 cells in DMEM-10% FBS more penicillin-streptomycin in vitro at 37 °C, 95% humidity and 5% CO2 on the seventh day, they were infected with T. cruzi epimastigotes TcV strain, isolated from patients with acute Chagas and preliminarily grown in biphasic media as NNN and LIT. Results: after 14 days of infection was observed the parasite forms: extracellular) that were released into the infected cells once lysed. Subsequent sub 3T3 cell cultures trypomastigotes obtained from epimastigotes obtaining improved T. cruzi-TcV. Conclusions: it is possible to obtain trypomastigotes from a local strain epimastigotes recreating the life cycle of the parasite in vitro.

Keywords : cell culture; epimastigotes; trypomastigotes; 3T3 cell; strain of T. cruzi - TCV.

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