Abstract

BUSTAMANTE GARCIA, Zulema et al. Microbiological molecular identification and serologic of Streptococcus pneumoniae and determination of the suceptibility to penicilin and eritromicin in the city of Cochabamba. Gac Med Bol [online]. 2009, vol.32, n.2, pp.29-34. ISSN 1012-2966.

Streptococcus pneumoniae is one of the main pathogens that cause breathing and invasive infections, that is why is important a quick identification of this microorganism and determine the sensitivity of the frequently used antibiotics, to execute appropriate therapies. With this purpose the identification of 32 isolates of Streptococcus pneumoniae from patients with breathing and invasive infections were carried out, conventional microbiological tests were applied for identification and for confirmation were used molecular techniques based on the chain reaction of polymerase (PCR), amplifying the regions of lyt A and ply genes and serological methods for the determination of the capsular antigen. Antibiograms were carried out in order to determinate the sensitivity to penicillin and erythromycin. The results showed a higher amount of isolates in children under 10 and adults over 49, and according to seasons, more isolates on winter months. Regarding sensitivity to antibiotics, we found a drop of 46.88% to penicillin, a drop of 6.25 to erythromycin and an intermediate drop of 15.63%. The statistical analysis among the three techniques used for reconfirmation revealed there is a low correlation among them. Therefore, optochin, used as a re-confirmation method, is lower than serological and molecular tests.

Keywords : Streptococcus pneumoniae; identification; lyt A; ply; capsular antigen; sensitivity penicillin; erythromycin.

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