Abstract

SANTA CRUZ RODRIGUEZ, Adriana C.; FIGUEROA VACA, Doris E.  and  DALENCE CONDORI, Rioan R.. COMPARACION DE DOS METODOS SEROLOGICOS PARA EL DIAGNOSTICO DE TOXOPLASMOSIS. Gac Med Bol [online]. 2007, vol.30, n.2, pp.11-14. ISSN 1012-2966.

The toxoplasmosis is an illness caused by the parasite Toxoplasma gondii, able to be transmitted to the fetus with serious consequences. For the toxoplasmosis diagnosis it is necessary to detect inmunoglobuline G (IgG) and M (IgM). Because ELISA gives an excessive number of false positive and false negative, we evaluate the utility of ELISA reagents for IgG and IgM detection at Hospital Elizabeth Seton in order to compare with Toxoplasma gondii inmnofluorescencia (IF) method used as reference. IgG (146 patients) and IGM (142 patients) were determined by ELISA and IF on serums from fertile age and pregnant women, and children consulted from June 2004 to October 2005 at Caja Petrolera de Salud. Sensibility, specificity and the predictive positive (VPP) and negtive (VPN) values were determined for ELISA technique. ELISA sensibility for IgG is 75%; specificicity 84%, VPP 64%; VPN, 90%. By ELISA it were not detected antibodies in 25 % of infeted patients and in 16% they were false positive; there is 64% of probability that individuals with positive result been really affected by the infection and 90% of probability that subject with seronegativity been really seronegative. For the IgM; ELISA has sensibility of 40%, specificity of 96%, VPP, 71%, and VPN, 88%. That means that ELISA was not able to detect 60% of infected, and in 4% it was false positive; there was 71% of probability that the patient be really affected and 88% of probability that it be really seronegative. Therefore, the ELISA technique has very low sensibility mainly for IgM, parameter that together with the specificity, VPP and VPN, should be taken on count at the time of do the laboratory interpretation and clinical diagnostic.

Keywords : Toxoplasmosis; Serology; ELISA; IF; Sensibility; Specificity.

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