Abstract

MANZUR SORIA, María del Rosario; BUSTAMANTE, Zulema  and  GONZALES, Gerardo. PRESENCIA DE INTEGRONES Y SU RELACIÓN CON LA RESISTENCIA A ANTIMICROBIANOS EN CEPAS DE  Pseudomonas aeruginosa. Gac Med Bol [online]. 2007, vol.30, n.1, pp.5-12. ISSN 1012-2966.

Due to the fact that in our environment there is an absence of statistical data related to the incidence of infections produced by P. aeruginosa in our population and the resistance that it presents with respect to a variety of antimicrobial agents, this study aims basically at providing information and data about distribution and frequency (after having standardized the detection techniques) of the integrons classes 1 and 2, and their relationship with their multi resistance to antimicrobial agents. For this purpase, samples of P. aeruginosa were obtained from different laboratories and hospitals in the State of Cochabamba, which were tested for antimicrobial sensibility, as well as for growth of bacteria under selective pressure, DNA extraction and finally detection of integrons class 1 and 2 with specific primers. Using PCR, integrons class 1 and 2 were detected in 41 samples of P. aeroginosa, identifying these ones in 58.5% of the strains: 29.7% of those isolated, carried integrons class 1, 12.2% had integrons class 2, and 17.07% had integrons of both classes. The association between class 1 integrons and multiple resistance to antimicrobial agents was statistically significant (chi square = 0.0033) thus showing a strong correlation, as opposed to what happened with the association among class 2 integrons (chi square = 0.8528) The authenticity of the products obtained from the PCR was confirmed through restriction tests made with SphI and HaeIII enzymes. This is the first report that shows prevalence of class 1 integrons and the relation with multi resistance aisolated strains of P. aeruginosa in Cochabamba, Bolivia.

Keywords : Integrons; Antimicrobial resistance; Conjugative plasmids.

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