Abstract

FLORES L., Amilcar A.  and  RODRIGUEZ H., Patricia. ESTANDARIZACIÓN DE LA PRUEBA DE ELISA PARA EL INMUNODIAGNÓSTICO DE HIDATIDOSIS HUMANA EMPLEANDO ANTÍGENOS DE PRODUCCIÓN LOCAL. Gac Med Bol [online]. 2006, vol.29, n.1, pp.5-10. ISSN 1012-2966.

A quatitative enzime-linked immunosorbent assay (ELISA) for the immunodiagnosis of hidatidosis is described. The ELISA was standardized using Echinococcus granulosus cyst fluid antigen -ELISA obtained from Sheep Liver. (ELISA-HID) were used to screen for cyst hidatic-specific IgC antibidies in serum samples from 17 patients with surgycally confirmed hydatic disease, 26 serum samples of patients healthy and 9 samples of serum from patients whit other cestode infections or with an another illess were used as controls. The sensitivity and specificity the ELISA -HID was 100% and 96% respectively. We observed relatively frequent cross-reactions with other parasitic diseases (cysticecosis). Cestode- related cross-reactivity can be resolved by the complementary use of Taenia solium cysticercosis-specitic immunoblotting. No cross reacction were noted in patients infected with Hymenolepis nana, T. cruzy and T. gondii. The excellent sensitivity and specificity of the ELISA-HID make the assay a potentially usefull tool in screening for antibodies agains Equinococosis.

Keywords : Echinococcus granulosus; hidatidosis; cyst hidatidic; cross reaction; ELISA; antigen (Ag).

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