Abstract

CAMACHO, Verushka; SOTO, Roberto; GUZMAN, Héctor  and  VARGAS, Virginia A.. Cellulase production by microorganisms isolated from Laguna Blanca, Potosí-Bolivia. Rev. Bol. Quim [online]. 2021, vol.38, n.4, pp.148-154. ISSN 0250-5460.  https://doi.org/10.34098/2078-3949.38.4.2.

Bacterial strains isolated from water samples collected from Laguna Blanca, Potosi-Bolivia and deposited at Biotechnology Center, Cochabamba-Bolivia, were subjected to cellulase enzyme production studies. Among 71 strains, 32 were selected as positive in liquid medium using filter paper as sole carbon source. Nine strains reported higher cellulase activity and the effect of NaCl concentration and pH on microorganism growth was evaluated. Thus, 7 strains were identified as halophiles meanwhile 2 were halotolerant. According to the medium pH value, 5 strains were neutrophiles and 4 alkalotolerants. Strains coded as LB-4 and LB-8 were selected as the best enzyme producers. Furthermore, cellulase production using forest products and agro-industrial residues were positive for different types of paper and pine wood. Nevertheless, higher activity values were detected using banana peel and sugarcane bagasse. The strain LB-8 was selected as the best cellulase producer under the conditions used in this study with 2.774 U/ml enzyme activity. Phylogenetic analyses showed 99.99 % sequence similarity between strain LB-8 and Bacillus pumilus.

Keywords : Cellulases; Forest products; Agro-industrial waste; Banana peel.

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