Abstract

PARRA LIZARAZU, Carla D.; QUIROGA SELAEZ, Gabriela; GIMENEZ TURBA, Alberto  and  FLORES QUISBERT, Esther. Rice-generated Aspergillus spp., Aflatoxin b1, its detection and quantification by fluorometric and hplc methods . Rev. Bol. Quim [online]. 2018, vol.35, n.5, pp.134-145. ISSN 0250-5460.

ABSTRACT Aflatoxins are produced by toxigenic strains of Aspergillus spp as a part of their metabolic producís. Its toxicity causes severe health damage which makes them to be considered potent carcinogens. There are Aspergillus strains that in substrates such as rice (Oryza sativa) grow and produce mycotoxins at 30 °C. Two analytical methods for the detection and quantification of aflatoxin B1 were developed; calibration curves were constructed to validate these methods. Aflatoxin Bl production was develop by strain 114 QD (Aspergillus spp.) in a médium containing 50 and 20 g of rice emulating when possible the storage conditions during incubation period. The analysis of 50 and 20 g of rice extracts by high performance liquid chromatography (HPLC) gave average concentrations of aflatoxin Bl of 94, 93 and 24,64 ppb respectively. By fluorescence spectroscopy average concentrations of 133, 37 and 27, 23 ppb were determined. Aflatoxin Bl concentrations obtained by both analytical methods show a significant difference (P <0, 05) with respect to the amount of substrate, being higher the concentration of aflatoxin B1 with greater amount of rice in the médium, the sensitivity of these methods allows to detect this toxin from 6 ppb.

Keywords : Afíatoxin Bl; Aspergillus spp; Oryza sativa; Fluorometry; HPLC.

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