Abstract

ALEJANDRO SANCHEZ, Varela; RODRIGUEZ LUNA, Isabel C  and  GUO, Xian Wu. Review of the Characterization of Aeromonas spp. And its Clinical importance. Rev. Bol. Quim [online]. 2017, vol.34, n.5, pp.132-137. ISSN 0250-5460.

ABSTRACT Advances in Microbiology and applications of Molecular Biology, has been achieved an improvement, complementary to the classical techniques of bacterial identification, this also allowed the discovery or regrouping of bacterial genera and species, which had failed to be identified correctly, by biochemical and phenotypic methods, since these have some limitations such as false positives or low specificity. Among the molecular tools used to identify the Aeromonas genus, we have used sequencing from 16S rRNA gene, maintenance genes such as rpoD or gyrB, which have a greater discrimination power than the 16S rRNA gene, the technique of RFLP-PCR and more recently the technique of Mass Spectrometry has been increased by Laser Assisted Matrix Desorption-Ionization of Flight Time (MALDI-TOF MS) which is able to identify species of Aeromonas quickly and accurately. This is important because of the increasing evidence that these species are widely distributed in the environment and can cause a variety of infections in animals and humans.

Keywords : Aeromonas; Methods; Molecular; Gene; Specificity.

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