Abstract

DELGADO S.*, Pavel K  and  DELGADO S., Derly A. In vitro cytotoxicity evaluation: DNA fragmentation in murine thymocytes by organophosphate dimethoate. Rev. Bol. Quim [online]. 2016, vol.33, n.1, pp.01-06. ISSN 0250-5460.

Studies on the effect of pesticides on health are of great interest because they can affect many organ systems such as the immune system which is very sensitive to exposure to environmental contaminants. Therefore, this paper presents the in vitro evaluation of the cytotoxicity and the DNA fragmentation in murine thymocytes , cultivated in vitro in different concentrations of organophosphate (0, 05 %, 0, 1 % and 0, 2 %) at intervals of 1, 3 and 6 hours respectively. The effect on the cell cytotoxicity, as well as the DNA damage was assessed. On the basis of the results obtained, it is concluded that dimethoate produces cytotoxicity, the generation of a superoxide anion radical which depends on the concentration and on the time. On the other hand it induces the generation of nitric oxide significantly for the concentrations: 0, 1 % and 0, 2 %. Electrophoretic evaluation of DNA after 6 hours of incubation showed a nucleosomal DNA fragmentation accompanied of a sweep, indicative of necrotic cell death.

Keywords : Citotoxicity; DNA Fragmentation; Dimethoate; Murine thymocites.

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