Abstract

GANDARILLAS, Cecilia; SOTO, Roberto  and  VARGAS, Virginia A. XYLANASE PRODUCTION USING BARLEY STRAW BY BACILLUS SP. LB-4 ISOLATED FROM LAGUNA BLANCA, POTOSI-BOLIVIA. Rev. Bol. Quim [online]. 2012, vol.29, n.1, pp.63-70. ISSN 0250-5460.

ABSTRACT  Bacterial strains isolated from water samples collected from Laguna Blanca, Potosi-Bolivia and deposited at Biotechnology Center, Cochabamba-Bolivia, were subjected to xylanolytic enzyme production studies. Among these, 20 strains were selected as positive in xylanolytic enzyme production in liquid medium, using barley straw as sole carbon source and subjected to morphological and biochemical studies. Enzyme production in liquid medium identified strain LB-4 as the best enzyme producer with 10 U/ml xylanolytic activity. Partial characterization of the produced enzyme revealed its display maximum activity values at pH 9, 50°C y 0% (w/v) NaCl concentration, thus exhibiting thermo-alkalitolerant properties. The 16S rDNA gene sequence analysis showed 99% similarity between strain LB-4 and Bacillus pumilus (embAJ494727).

Keywords : xylanolytic activity; barley straw; Bacillus sp.

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