Abstract

QUITIGUINA, Carla  and  SANTACRUZ, Stalin. OBTENCIÓN DE JARABE DE GLUCOSA A PARTIR DE LA HIDRÓLISIS ENZIMÁTICA DE ALMIDÓN DE BANANO, MUSA CAVENDISH. Rev. Bol. Quim [online]. 2012, vol.29, n.1, pp.55-62. ISSN 0250-5460.

ABSTRACT  Banana (Musa cavendish) is a fruit that takes place in Ecuador and a part of its production is being rejected. For this reason this project is focused on the usage of this resource in order to obtain glucose syrup from its starch by an enzymatic pathway. Previous to hydrolysis the substrate needs to be prepared using a process of washing and drying of banana fruits. Hydrolysis was made in two stages. For the first hydrolysis ± -amylase from Bacillus amyloliquefaciens was used; which allowed to degrade starch to dextrins. In this stage some tests were made to calculate the optimal time for the hydrolysis, which was of 2 hours. Also, dextrins obtained were analyzed by gel permeation chromatography, obtaining dextrins of 9, 10 and 11 glucose units. In the second stage amyloglucosidase from Aspergillus niger was used. In this stage an experimental design was performed; such design was completely at random with factorial model of 3², corresponding to the combination of time and amount of enzyme, with two repetitions. The studied variables were yield and crystallinity. The recommended treatment was of 1,5 milliliters of enzyme and 14 hours of hydrolysis. Treatment that corresponds to a glucose yield of 90,14%.

Keywords : hidrólisis del almidón; banano; ± -amilasa; amiloglucosidasa.

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