Introduction:

To determine the resistance profile of microorganisms isolated from pediatric patients treated at the Manuel Ascencio Villarroel Children's Hospital, Cochabamba, Bolivia.

Materials and methods:

A quantitative, descriptive and correlational study was carried out in 97 pediatric patients. For the analysis, biochemical tests and antibiograms were used following the standards established by the Clinical and Laboratory Standards Institute (CLSI).

Results:

Escherichia coli was the most frequent microorganism in the isolates, with 28,9%, followed by Burkholderia cepacia with 21,6% and Pseudomonas aeruginosa with 11,3%. Escherichia coli showed high resistance to ampicillin, cefazolin and sulfatrimethoprim, while Pseudomonas aeruginosa showed notable resistance to imipenem and ceftazidime. For its part, Burkholderia cepacia showed resistance to both ceftazidime and meropenem. The production of extended-spectrum beta-lactamases (ESBL) in Escherichia coli (16,5%) and cAMP in Pseudomonas aeruginosa (8,2%) were identified as resistance mechanisms.

Discussion:

The findings show a high rate of resistance to commonly used antibiotics, suggesting caution in empiric therapy and continued surveillance for antimicrobial resistance.