Abstract

DO AMARAL CRISPIM, Bruno et al. Evaluation protocols for the extraction of genomic DNA from Bovine blood. J. Selva Andina Res. Soc. [online]. 2016, vol.7, n.2, pp.95-103. ISSN 2072-9294.

Basic studies on DNA extraction techniques are very important for the success of scientific papers in the field of molecular biology. The extraction and purification of nucleic acids are critical steps for establishing further genetic analysis. The objective of this study was to evaluate the efficacy of different protocols for DNA extraction by determining the quantity and quality of extracted genetic material and the possibility of amplification by PCR. We did DNA extraction and PCR of ten bovine blood samples. The test results obtained by spectrophotometry indicated that the quantity and quality of genomic DNA were considered satisfactory in all protocols for PCR. However, there was a statistically significant difference between the parameters measured, both in quantity and in quality (p <0.01). The extraction protocol using whole blood was more efficient in terms of time and quality; there was no degradation in all processes of extraction. It was also demonstrated that the possibility of amplification of the region of exon 2 of the leptin gene in extracted DNA exists.

Keywords : Test extraction; PCR; bovine; molecular biology.

        · abstract in Spanish     · text in Portuguese     · Portuguese ( pdf )